Point-of-Care Tests via Pollen-Based Nanoplasmonic Chips Combined with a Synthetic Receptor for FKBP12 Biomarker Detection at a Single-Molecule Level

Marzano, C., Pitruzzella, R., Bartolini, C., Pasquardini, L., Grasta, A.L., Tozzetti, M., Germinario, R., Natale, T., Ishaq, A., Menichetti, S., Arcadio, F., Zeni, L., Dell’Olio, F., Caminati, G., Cennamo, N. (2025) ACS Applied Nano Materials

The selective and rapid detection of FKBP12 is crucial due to its involvement in immunosuppression, neurodegenerative and oncological diseases, and some fundamental cellular processes. A low-cost point-of-care test (POCT) based on a simple setup, combined with plasmonic sensor chips for ultrasensitive detection of FKBP12, is developed. The sensing principle exploits pollen-based natural nanostructures covered by gold nanofilms and functionalized with synthetic GPS-SH1 receptors. The experimental results demonstrated ultrahigh performance due to…

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